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Part:BBa_K2550002

Designed by: Janet Standeven   Group: iGEM18_Lambert_GA   (2018-10-10)


T7 Cholera ptxb1 Toehold LacZ

Toehold switches are riboregulators that are hairpin structures that open to expose the Ribosomal binding sites located in the loop when their corresponding RNA binds to a complementary sequence. This switch was designed to recognize the sequence located within the CtxB1 gene from V. cholera. This gene is non-toxic and specific to only pathogenic strains of V. cholera. This switch is to be used with the complementary trigger part BBaK2550003.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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